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- Clone
- HK1.4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Lymphocyte antigen 6 complex, locus C
- Isotype
- Rat IgG2c, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Compare all formats See APC/Cyanine7 spectral data
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
128025 | 25 µg | 104⬠| ||||
128026 | 100 µg | 249⬠|
Description
Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.
Product Details
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- L3 cloned CTL cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
- FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Clone HK1.4 does not block the binding of clone RB6-8C58.
Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.
- Additional Product Notes
- BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation)
-
- Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
- Herold KC, et al. 1990. Diabetes 39:815. (Activ)
- Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
- Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
- Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
- Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
- Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
- Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
- Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
- Product Citations
-
- Parsons M, et al. 2014. J Immunol. 192:1361. PubMed
- Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
- He J, et al. 2022. Front Immunol. 12:760138. PubMed
- Ippagunta S, et al. 2016. Proc Natl Acad Sci U S A. 113: E6162 - E6171. PubMed
- Rodriguez-GarcÃa A, et al. 2021. Nat Commun. 12:877. PubMed
- Súarez-López L, et al. 2021. iScience. 24:103406. PubMed
- Sun CP, et al. 2021. PLoS Pathog. 17:e1009758. PubMed
- Abdala-Valencia H, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:482. PubMed
- Kim AS, et al. 2021. Cell. 184(17):4414-4429.e19. PubMed
- Heng Y, et al. 2020. Glia. . PubMed
- Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
- Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
- de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
- Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
- Tanner MA, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H633. PubMed
- McDowell SAC, et al. 2021. Nat Cancer. 2:545. PubMed
- Zhou HF, et al. 2022. Front Pharmacol. 13:778755. PubMed
- Royzman D, et al. 2022. Front Immunol. 13:1012647. PubMed
- Lin T, et al. 2015. PLoS One. 10: e0138335. PubMed
- Wada N, et al. 2020. Mol Metab. 37:100988. PubMed
- Motoyama S, et al. 2020. Cells. :9. PubMed
- Chan YH, et al. 2019. EMBO Mol Med. 11:e10092. PubMed
- Walker FC, et al. 2021. PLoS Pathog. 17:e1009402. PubMed
- Movita D, et al. 2015. J Virol. 89:4809. PubMed
- Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
- Hou X, et al. 2022. Cell Death Dis. 13:100. PubMed
- Fujita K, et al. 2019. Proc Natl Acad Sci U S A. 116:14714. PubMed
- Yu S, et al. 2016. Mol Ther. 10.1038/mt.2016.175. PubMed
- Yin S, et al. 2016. Nat Commun. 7: 11311. PubMed
- Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
- Wang Y, et al. 2021. Cell Rep. 35:108948. PubMed
- Formaglio P, et al. 2021. Immunity. 54:2724. PubMed
- Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
- Abdul Pari AA, et al. 2020. Cancer Res. 80:2586. PubMed
- Lim HY et al. 2018. Immunity. 49(2):326-341 . PubMed
- Guérin MV, et al. 2019. Nat Commun. 10:4131. PubMed
- , et al. 2021. Eur J Immunol. 51:2708. PubMed
- Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
- McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
- Bambouskova M, et al. 2021. Cell Reports. 34(10):108756. PubMed
- AM C, et al. 2015. Proc Natl Acad Sci U S A. 112 7557 . PubMed
- Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
- Kuhn JA, et al. 2021. Elife. 10:. PubMed
- Lin J, et al. 2021. Nat Commun. 12:5321. PubMed
- Terauchi R, et al. 2021. PLoS One. 16:e0239108. PubMed
- Wang SK, et al. 2021. JCI Insight. 6: . PubMed
- Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed
- Grauel AL, et al. 2020. Nat Commun. 4.84375. PubMed
- Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
- Maisel D, et al. 2016. PLoS One. 11: 0159716. PubMed
- Simões FC, et al. 2020. Nat Commun. 0.875. PubMed
- Luo J et al. 2018. Immunity. 49(1):107-119 . PubMed
- Shepardson K, et al. 2016. MBio. 7: e00506-16. PubMed
- Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
- Mossadegh–Keller N, et al. 2019. Bio Protoc. 9:5. PubMed
- Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
- Olson B, et al. 2021. Brain Behav Immun. 97:102. PubMed
- Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
- Capuccini B, et al. 2016. Sci Rep. 6:39258. PubMed
- de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
- Walker MT, et al. 2021. Front. Allergy. 2:. PubMed
- Mulens-Arias V, et al. 2022. Pharmaceutics. 14:. PubMed
- Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
- Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
- Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
- Buerfent BC, et al. 2019. GMS Infect Dis. 7:Doc04. PubMed
- Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
- Fedele C, et al. 2021. J Exp Med. 218: . PubMed
- Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
- Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
- van de Garde M, et al. 2016. PLoS One. 11:e0166094. PubMed
- Baker GJ, et al. 2020. Cell Syst. 0.647222222. PubMed
- Gardner A, et al. 2022. J Immunother Cancer. 10:. PubMed
- Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
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- Lynn GM, et al. 2020. Nat Biotechnol. 38:320. PubMed
- RRID
- AB_10643867 (BioLegend Cat. No. 128025)
AB_10640120 (BioLegend Cat. No. 128026)
Antigen Details
- Structure
- 14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure hom*ology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
- Distribution
-
Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.
- Cell Type
- Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97. - Gene ID
- 17067 View all products for this Gene ID
- UniProt
- View information about Ly-6C on UniProt.org
Documentation
- Technical Data Sheet (pdf)
- Certificate of Analysis
- Safety Data Sheet
Reviews
Review This Product
Related Protocols
- Cell Surface Flow Cytometry Staining Protocol
Related Products
Description | Clone | Applications |
---|
Pages
- Quality Control
- Multicolor Staining Guide
- Fluorescence Spectra Analyzer
- Fluorophore Brightness Index
- Custom Panel Construction
- Fluorophore Equivalency Tool
Related FAQs
There are no FAQs for this product.
Other Formats
View All Ly-6C Reagents Request Custom Conjugation
Description | Clone | Applications |
---|---|---|
Pacific Blue™ anti-mouse Ly-6C | HK1.4 | FC |
APC anti-mouse Ly-6C | HK1.4 | FC |
Purified anti-mouse Ly-6C | HK1.4 | FC,IHC-F |
Biotin anti-mouse Ly-6C | HK1.4 | FC |
FITC anti-mouse Ly-6C | HK1.4 | FC |
Alexa Fluor® 647 anti-mouse Ly-6C | HK1.4 | FC |
PE anti-mouse Ly-6C | HK1.4 | FC |
PerCP/Cyanine5.5 anti-mouse Ly-6C | HK1.4 | FC |
PE/Cyanine7 anti-mouse Ly-6C | HK1.4 | FC |
Alexa Fluor® 488 anti-mouse Ly-6C | HK1.4 | FC |
Alexa Fluor® 700 anti-mouse Ly-6C | HK1.4 | FC |
APC/Cyanine7 anti-mouse Ly-6C | HK1.4 | FC |
PerCP anti-mouse Ly-6C | HK1.4 | FC |
Brilliant Violet 570™ anti-mouse Ly-6C | HK1.4 | FC |
Brilliant Violet 421™ anti-mouse Ly-6C | HK1.4 | FC |
Brilliant Violet 510™ anti-mouse Ly-6C | HK1.4 | FC |
Brilliant Violet 605™ anti-mouse Ly-6C | HK1.4 | FC |
Brilliant Violet 711™ anti-mouse Ly-6C | HK1.4 | FC |
Purified anti-mouse Ly-6C (Maxpar® Ready) | HK1.4 | FC,CyTOF® |
Brilliant Violet 785™ anti-mouse Ly-6C | HK1.4 | FC |
PE/Dazzle™ 594 anti-mouse Ly-6C | HK1.4 | FC |
APC/Fire™ 750 anti-mouse Ly-6C | HK1.4 | FC |
TotalSeq™-A0013 anti-mouse Ly-6C | HK1.4 | PG |
Brilliant Violet 650™ anti-mouse Ly-6C | HK1.4 | FC |
TotalSeq™-C0013 anti-mouse Ly-6C | HK1.4 | PG |
TotalSeq™-B0013 anti-mouse Ly-6C | HK1.4 | PG |
APC/Fire™ 810 anti-mouse Ly-6C Antibody | HK1.4 | FC |
Spark UV™ 387 anti-mouse Ly-6C | HK1.4 | FC |
PE/Fire™ 810 anti-mouse Ly-6C | HK1.4 | FC |
Spark Blue™ 550 anti-mouse Ly-6C (Flexi-Fluor™) | HK1.4 | FC |
Certificate of Analysis
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For Research Use Only. Not for diagnostic or therapeutic use.
This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for userâs intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Pacific Blue™ anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells stained with HK1.4 Pacific B... -
APC anti-mouse Ly-6C
C57BL/6 bone marrow cells stained with HK1.4 APC -
Purified anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells stained with purified HK1.4,... Fresh, frozen mouse spleen was stained with purified Ly6C cl... -
Biotin anti-mouse Ly-6C
BALB/c bone marrow cells stained with HK1.4 biotin, followed... -
FITC anti-mouse Ly-6C
C57BL/6 bone marrow cells stained with HK1.4 FITC (gated on ... -
Alexa Fluor® 647 anti-mouse Ly-6C
BALB/c bone marrow cells stained with HK1.4 Alexa Fluor®... -
PE anti-mouse Ly-6C
C57BL/6 bone marrow cells were stained with Ly-6C (clone HK1... -
PerCP/Cyanine5.5 anti-mouse Ly-6C
C57BL/6 bone marrow cells stained with HK1.4 PerCP/Cyanine5.... -
PE/Cyanine7 anti-mouse Ly-6C
C57BL/6 bone marrow cells stained with HK1.4 PE/Cyanine7 -
Alexa Fluor® 488 anti-mouse Ly-6C
C57BL/6 bone marrow cells stained with anti-mouse Ly-6C, HK1... -
Alexa Fluor® 700 anti-mouse Ly-6C
-
APC/Cyanine7 anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells were stained with CD8a FITC ... -
PerCP anti-mouse Ly-6C
C57BL/6 bone marrow cells were stained with Ly-6C (clone HK1... -
Brilliant Violet 570™ anti-mouse Ly-6C
C57BL/6 bone marrow cells were stained with Ly-6C (clone HK1... -
Brilliant Violet 421™ anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells were stained with Ly-6C (clo... -
Brilliant Violet 510™ anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells were stained with Ly-6C (clo... -
Brilliant Violet 605™ anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells were stained with Ly-6C (clo... -
Brilliant Violet 711™ anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells were stained with Ly-6C (clo... -
Purified anti-mouse Ly-6C (Maxpar® Ready)
Mouse splenocytes stained with 150Nd-anti-Ly-6C (HK1.4) and ... -
Brilliant Violet 785™ anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells were stained with Ly-6C (clo... -
PE/Dazzle™ 594 anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells were stained with Ly-6C (clo... -
APC/Fire™ 750 anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells were stained with Ly-6C (clo... -
TotalSeq™-A0013 anti-mouse Ly-6C
-
Brilliant Violet 650™ anti-mouse Ly-6C
C57BL/6 mouse splenocytes were stained with CD8 FITC and Ly-... -
TotalSeq™-C0013 anti-mouse Ly-6C
-
TotalSeq™-B0013 anti-mouse Ly-6C
-
APC/Fire™ 810 anti-mouse Ly-6C Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse CD8a ... -
Spark UV™ 387 anti-mouse Ly-6C
C57BL/6 mouse splenocytes were stained with anti-mouse CD8a ... -
PE/Fire™ 810 anti-mouse Ly-6C
C57BL/6 mouse splenocytes were stained with anti-mouse CD8a ... -
Spark Blue™ 550 anti-mouse Ly-6C (Flexi-Fluor™)